In a recent article published in Plant and Cell Physiology, the team led by Kamel Hammani, which investigates the mechanisms of chloroplast gene expression, demonstrates that synthetic PPR-type RNA-binding proteins reduced to their modular core are sufficient to stabilize chloroplast RNAs in vivo. This work shows that the terminal domains traditionally added to these proteins are not essential, thereby simplifying the design of molecular tools for manipulating gene expression in plant organelles.
Simplifying the engineering of RNA-binding proteins in chloroplasts