Ins and outs of viral infection in plants

Group leaders : Véronique ZIEGLER-GRAFFDavid GILMER

Research area

Early during viral infections, specific and non-specific interactions take place between both viral and host factors, leading to either progression or arrest of the infection. In particular mechanisms of plant defence can be countered by viruses encoding various pathogenicity factors. These viral factors can act by interfering with the innate defence pathways like RNA silencing or by deregulating the hormone signalling pathways to restrain or block the infection process. Our studies focus on the molecular mechanisms underlying pathogenicity, suppression of RNA silencing and viral movement as these viral functions appear to be closely coordinated during the multiplication cycle. The viruses studied belong to the Benyvirus and Polerovirus genera whose strategies of expression, spreading in the plant and vector-borne transmission are distinct.

Projects

Polerovirus movement in the phloem

Project manager: Véronique ZIEGLER-GRAFF

Poleroviruses are characterized by their obligate transmission by aphids and their phloem tropism. Recently we discovered a new protein P3a encoded by a cryptic gene conserved among poleroviruses. This small protein expressed from a non-conventional initiation codon is essential for viral long distance movement in the plant. Our objective is to decipher the mode of action of this small protein, in particular its interactions with the other viral proteins involved in viral movement.

Role of the polerovirus silencing suppressor P0 protein in the viral infection process

Project manager: Véronique ZIEGLER-GRAFF

The RNA silencing suppressor P0 functions by inducing the degradation of the protein ARGONAUTE 1, the key effector of this antiviral defence pathway. This leads to a number of gene deregulations potentially involved in the establishment of infection. Such deregulations could also play a role in virus transmission by the aphid vector. This hypothesis is investigated in collaboration with the team of Dr. Véronique Brault at INRA of Colmar (ANR VIRAPHIPLANT). In parallel we further investigate the role of P0 by seeking new cellular partners and by site-directed mutagenesis.

Role of the viral non-coding RNA in BNYVV infection

Project manager: David GILMER

BNYVV (Beet necrotic yellow vein virus) is the etiological agent of Rhizomania, a viral disease that induces massive root proliferation in sugar beet. Depending on the isolates, the viral genome consists of four to five single stranded RNAs of positive polarity. RNA-3 undergoes cleavage leading to the accumulation of a non-coding RNA (ncRNA) stabilized by the “coremin” sequence present on RNA-3. Both ncRNA and BNYVV silencing suppressor p14 protein are essential to viral systemic spread in the plant. Current studies aim at understanding the complex interplay between long-distance movement and RNAi.

Preservation of BNYVV genome integrity during systemic infection

Project manager: David GILMER

The four to five genomic BNYVV RNA are packaged in separate rod shape particles. We are exploring the mechanism whereby all viral RNA can reach the same cell to initiate a new replication cycle after long distance movement in the vasculature. Preliminary data obtained in collaboration with Dr. Claudio Ratti (Bologna, Italy) suggest the existence of molecular interactions between viral RNAs. We are currently characterizing these interactions.

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Synergism between BNYVV and BMYV (Polerovirus)

Project manager: David GILMER

Co-infections of sugar beet by both BNYVV and Beet mild yellowing virus (BMYV, Polerovirus) are occurring in the field in natural conditions and the plants develop more severe symptoms compared to single infections. In collaboration with the breeding company SESVanderHave we are investigating the potential synergism between the two viruses and the consequences for sugar beet production.

Members

Selected publications

  • GILMER D., RATTI C. and MICHEL F.

    Long-distance movement of helical multipartite phytoviruses: keep connected or die?

    Current Opinion in Virology, 33:120-128, 2018.

  • FLOBINUS A., CHEVIGNY N., CHARLEY P., SEISSLER T., KLEIN E., BLEYKASTEN-GROSSHANS C., RATTI C., BOUZOUBAA S.E., WILUSZ J. and GILMER D.

    Beet Necrotic Yellow Vein Virus Noncoding RNA Production Depends on a 5' to 3' Xrn Exoribonuclease Activity

    Viruses, 10(3):137, 2018. | DOI : 10.3390/v10030137DOI logo

  • BORTOLAMIOL-BÉCET D., MONSION B., CHAPUIS S., HLEIBIEH K., SCHEIDECKER D., ALIOUA A., BOGAERT F., REVERS F., BRAULT V. and ZIEGLER-GRAFF V.

    Phloem-triggered virus-induced gene silencing using a recombinant polerovirus

    Frontiers in Microbiology, 9:2449, 2018. | DOI : 10.3389/fmicb.2018.02449DOI logo

  • DALL'ARA M., RATTI C., BOUZOUBAA S.E. and GILMER D.

    Ins and Outs of Multipartite Positive-Strand RNA Plant Viruses: Packaging versus Systemic Spread

    Viruses, 8(228):1-18, 2016. | DOI : 10.3390/v8080228DOI logo

  • SMIRNOVA E., FIRTH A.E., MILLER W.A., SCHEIDECKER D., BRAULT V., REINBOLD C., RAKOTONDRAFARA A.M., CHUNG B.Y.W. and ZIEGLER-GRAFF V.

    Discovery of a small Non-AUG-Initiated ORF in Poleroviruses and Luteoviruses that is required for long-distance movement

    PLoS pathogens, 6(11):e1004868, 2015. | DOI : 10.1371/journal.ppat.1004868DOI logo