RNA degradation stands among the most powerful processes to control gene expression. Diverse and intricate RNA decay pathways cooperate:
- to ensure that the degradation of coding and non-coding RNAs is tightly regulated in response to developmental or environmental stimuli
- to eliminate defective transcripts (RNA quality control)
- to counterbalance loose transcriptional control by degrading transcripts generated from intergenic regions (RNA surveillance)
- to fight pathogens such as viruses.
Our main objectives are to identify key actors of RNA degradation pathways in plants, and to determine their impact on genome expression, development or stress response. We focus on new co-factors of the RNA exosome, the main 3’-5’ exoribonucleolytic complex in Eukaryotes, and on components of the non-sense mediated decay pathway. We aim to understand their roles in RNA substrate recognition and degradation. We are also particularly interested in a class of enzymes that adenylate or uridylate RNAs and we study how these 3’ modifications impact RNA’s fate. Finally, we address the roles of RNA degradation pathways during RNA virus infections.
New components of RNA decay and their functions are identified through forward and reverse genetics strategies in the model plant Arabidopsis thaliana, or by protein biochemistry approaches coupled to mass spectrometry analyses. We also develop new high-throughput techniques to identify 3’ modifications of transcripts.
Key fundings of our current research include the NetRNA LabEx (ANR-2010-LABX-36 ; 2011-2020) and the 3’modRN ANR grant (ANR-15-CE12-0008 ; 2015-2020).